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Tour Guide Barbie is a Barbie doll initially from Al's Toy Barn in Toy Story 2. She and all of the other Barbie dolls in the film are voiced by Jodi Benson. When Hamm, Slinky Dog, Rex and Mr. Potato Head come upon the Barbie aisle at Al's Toy Barn while searching for Woody, Tour Guide Barbie hops into the toy car they are driving. She gives the toys a tour in the toy barn and helps them locate Buzz Lightyear (they actually find a "Bonus Belt" Buzz; see below).

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